Gene Engineering Technology Platform

♦ Modular Gene Vector Design & Construction Platform

This platform enables diverse gene vector designs tailored to research needs, ranging from basic plasmid construction to complex multi-gene circuit assembly, providing tools and workflows at multiple levels.

• Restriction Enzyme Cloning & TA Ligation: Standardized DNA subcloning and PCR product preparation for sequencing.

• Multi-Fragment Seamless Assembly: Based on homology-based recombination, allowing simultaneous assembly of multiple DNA fragments; ideal for large expression vectors or complex metabolic pathways.

• Site-Specific Recombination: Uses standardized recombinase systems for efficient, directional transfer of DNA modules between vectors, suitable for high-throughput vector construction.

 

♦ Cell Line Genome Engineering Platform

This platform offers various gene modification strategies, covering workflows from short-term exogenous gene expression to long-term genomic modification. Capabilities include gene knockout, knock-in, and point mutations. Project-specific evaluations balance speed, stability, and controllability, with corresponding screening and validation workflows to ensure optimal research outcomes.

• Transient Gene Expression System: Enables short-term, high-level expression of exogenous genes without permanently altering the genome. Primarily used for rapid gene function screening and small-scale protein production.
• Random Integration System: Designed to establish stable expression cell lines through random integration, generating numerous integration events quickly, followed by selection of high-expressing clones. Suitable for long-term stable expression or early production line development.
• Targeted Integration System: Employs nuclease-guided technologies for precise genomic modification at specific loci (e.g., Safe Harbor loci), reducing uncertainties from random insertion and improving expression consistency across clones.

• Functional validation of drug targets
• Analysis of cell signaling mechanisms
• Generation of cell models for human genetic diseases
• Genetic modification of cells
• Development of recombinant proteins and monoclonal antibodies
• Gene design and validation in synthetic biology

• Academic Research Institutions
• Biotechnology and Pharmaceutical R&D Units

Built on modular and standardized gene manipulation workflows, this platform transforms traditionally complex vector construction and genome modification processes into controlled and highly efficient steps.

In addition to reducing errors caused by randomness, it provides stable and consistent experimental models, minimizing the need for repeated adjustments in subsequent research.

With this workflow design, researchers can obtain reproducible and verifiable results in a shorter timeframe, laying a reliable foundation for downstream functional studies, application development, and industrial translation.