This service provides recombinant protein expression and purification using yeast as the host, suitable for applications such as research protein production, antigen development, and activity validation. The yeast system combines the protein modification capabilities of eukaryotes with high-efficiency cultivation, making it an ideal choice between bacterial and mammalian cell systems. Protein production can be carried out using systems such as Pichia pastoris or Saccharomyces cerevisiae, depending on your requirements.
SERVICE
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Yeast Recombinant Protein Expression & Purification
Service Description
- Small-scale pilot experiments to optimize expression conditions
- Affinity chromatography purification (common tags such as His, Strep, FLAG)
- Protein concentration, buffer exchange, and sterile filtration
- Basic purity verification and concentration analysis
Service Workflow
Requirement Discussion & Plan Design
Protein Expression Condition Testing & Optimization
Protein Purification & Concentration Processing
Quality Verification
Data Reporting & Recombinant Protein Delivery
Sample/Specification Requirements
- Recommended to include common purification tags such as His, FLAG, or Strep
- Provide sequence information (at minimum the ORF region) and construct background information
- Notify in advance if there are special requirements (e.g., secretory protein, co-transfection with helper elements, etc.)
Shipping Specifications / Acceptance Criteria
Purified Protein: Delivered as requested, in frozen form
Accompanying Report: SDS-PAGE images, protein concentration