This service provides recombinant protein expression and purification using mammalian cells as hosts, suitable for producing proteins that require proper folding, glycosylation, or other mammalian-specific post-translational modifications. It assists clients in obtaining high-quality, biologically active target proteins, suitable for downstream functional studies, antibody production, or product development.
SERVICE
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Recombinant Protein Expression and Purification in Animal Cells
Service Description
- Small-scale pilot experiments to optimize conditions (cell lines, culture parameters, etc.)
- Affinity chromatography purification (common tags such as His, Strep, FLAG)
- Protein concentration, buffer exchange, and sterile filtration
- Basic purity verification and concentration analysis
Service Workflow
Consultation & Project Design
Protein Expression Testing & Optimization
Protein Purification & Concentration
Quality Verification
Data Reporting & Delivery of Recombinant Protein
Sample/Specification Requirements
- Recommended to include common purification tags such as His, FLAG, or Strep
- Provide sequence information (at minimum the ORF region) and construct background details
- Notify in advance of any special requirements (e.g., secreted proteins, co-transfection with helper elements, etc.)
Shipping Specifications / Acceptance Criteria
Purified Protein: Delivered as requested, typically in frozen form
Accompanying Report: Includes SDS-PAGE images, protein concentration, total yield, and a brief summary of operational conditions