We provide technical services in microbial genetic engineering, focusing on plasmid construction, microbial genome editing, and transformation procedures. Depending on the microbial species and research objectives, we design and execute strategies such as gene introduction, homologous recombination, and targeted editing to support clients’ development needs in protein expression, metabolic engineering, and functional analysis.
SERVICE
Cell-Based Drug Screening Services
Gene Engineering Services
Laboratory Mouse Import Agency Services
Gene Engineering Services
Bacterial and Yeast Genetic Engineering Services
Service Description
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Plasmid Construction
Design modular plasmids based on expression requirements, supporting combinations of promoters, selection markers, fusion tags (e.g., His-tag, GFP), and antibiotic resistance genes. Suitable for various expression systems and analytical purposes.
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Microbial Transformation and Gene Introduction
For common strains (e.g., E. coli, B. subtilis, S. cerevisiae, P. pastoris), perform plasmid transformation or homologous recombination to introduce target sequences, enabling foreign gene expression, knock-in, or simple functional simulations.
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Gene Editing Design and Execution
Carry out gene knockout, point mutation, or marker insertion in bacterial or yeast strains. Services include guide RNA design, vector construction, homologous repair template planning, and transformation procedures, applicable for functional gene analysis, metabolic pathway engineering, or reporter system development.
Service Workflow
Consultation and Project Planning
Strain Condition Testing
Plasmid Construction and Nucleic Acid Synthesis
Microbial Transformation and Gene Introduction Assays
Genotype Analysis and Sequencing Verification
Data Reporting and Final Product Delivery
Sample/Specification Requirements
- Target gene sequence or functional fragment information (including mutation sites or insertion points)
- Any specified plasmid or reporter gene
- Exogenous gene sequence and related regulatory elements (e.g., promoters)
Shipping Specifications / Acceptance Criteria
- Completed plasmid constructs
- Successfully transformed microbial strains
- Genotype verification data
Note: This service is not intended for clinical diagnostics, therapeutic use, or any other medical applications.