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Gene Engineering Services

Generation of Gene-Edited Mouse Models

We provide professional and customized services for generating gene-edited mouse models, assisting clients with various genetic engineering approaches such as knockout (KO), knock-in (KI), and point mutations of target genes. From experimental design and gene editing to the delivery of mature mice, we employ a fully integrated, one-stop workflow to maximize editing efficiency and success rate. This service is suitable for applications including disease mechanism studies, drug development, and functional gene investigations.

Service Description

  • Gene Knockout (KO)

    Using targeted gene editing and embryo microinjection techniques, specific gene fragments are precisely removed to generate functional loss models. This approach is suitable for studying gene functions under physiological and pathological conditions and is widely applied in target validation and basic biomedical research.

  • Gene Knock-in (KI)

    Exogenous sequences, such as fluorescent reporter genes (e.g., GFP), drug-inducible genes, or larger DNA fragments (e.g., BACs), are inserted at specific genomic loci. Applications include monitoring protein expression, tracking cellular activity, generating humanized animal models, or establishing conditional systems (e.g., Cre/loxP and FLP/FRT) to enable spatial and temporal control in research.

  • Point Mutation

    Single-nucleotide variations are introduced at precise genomic positions to model human disease-causing mutations. This strategy is suitable for studying pathogenic mechanisms and supporting personalized medicine development.

 

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Service Workflow
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Consultation and Project Planning
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Design of Editing Tools / Vectors
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Embryo Microinjection
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Embryo Transfer into Recipient Females
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Birth of Embryos and Initial Screening
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Genotyping and Sequencing Verification
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Data Reporting and Delivery of Mature Mice
Sample/Specification Requirements
  • Target gene information (including mutation sites or insertion design)
  • For knock-in projects, please provide the complete insertion sequence (including promoter or reporter gene)
  • If using a custom vector or reporter system, please provide details during consultation
  • If Cre/LoxP, FLP/FRT expression systems, or conditional designs are required, please specify the relevant mouse strains
Shipping Specifications / Acceptance Criteria
  • Mouse strains: Several F0/F1 generation mice (as per contract)
  • Strain background: C57BL/6 or B6D2F1 (customizable)
  • Acceptance items:
    • – PCR genotyping report
    • – Sanger sequencing verification report
    • – Animal husbandry records
Note: This service is not intended for clinical diagnostics, therapeutic use, or any other medical applications.